نتایج جستجو برای: genomic DNA
تعداد نتایج: 582362 فیلتر نتایج به سال:
background: nematodes are among the most common and important parasites of man and animal. dna of a single worm can be used for several purposes, such as identification to the species, subspecies, strain and antihelmenthic resistance. dna extraction from a single small worm using traditional methods such as phenol extraction technique faces serious prob lems. methods: dna from 20 single haemonc...
background: extraction of blood genomicdnais one of the main approaches for clinical and molecular biology studies. although several methods have been developed for extraction of blood genomic dna, most of these methods consume long time and use expensive chemicals such as proteinase k and toxic organic solvent such as phenol and chloroform. the objective of this study was to developed easy and...
Genomic DNA extraction with a high quantity and quality is a basic requirement in molecular biology. The DNA obtained was free of any contamination proteins, polysaccharide, polyphenols and colored pigments. These compounds would interfere with the genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The isolated genomic DNA was fo...
بر خلاف مرغ که اطلاعات ژنومی آن کامل است، اطلاعات ژنتیکی زیادی در رابطه با بلدرچین در دسترس نیست. با توجه به شباهت¬های ژنتیکی بلدرچین ژاپنی با مرغ و تکمیل نقشه ژنومی مرغ، توالی ژنومی تطبیقی آن و همولوژی مرغ با از جمله بلدرچین می تواند مورد مطالعه قرار بگیرند و نقشه ژنومی آن تهیه شود. هدف از این تحقیق بررسی همسانی ژنوم بلدرچین ژاپنی و مرغ بر اساس توالی dna بود. برای این منظور بخشی از توالی dna ژ...
In the present study bacteriophages isolated from rainbow trout farms and sewage treatment plants were genetically identified and their effectiveness on Yersinia ruckeri isolates from clinical cases of red mouth disease was investigated. Fish samples suspected to red mouth disease were collected from rainbow trout farms located in west Azerbaijan. Y. ruckeri, the causative agent of red mouth d...
In the present study bacteriophages isolated from rainbow trout farms and sewage treatment plants were genetically identified and their effectiveness on Yersinia ruckeri isolates from clinical cases of red mouth disease was investigated. Fish samples suspected to red mouth disease were collected from rainbow trout farms located in west Azerbaijan. Y. ruckeri, the causative agent of red mouth d...
Abstract Background and objectives: Genomic DNA extraction of bacterial cells is of processes performed normally in most biological laboratories therefore, various methods have been offered, manually and kit, which may be time consuming and costly. In this paper, genomic DNA extraction of Staphylococcus aureus was investigated using some laundry detergent brands available in Iran to achieve ...
نقطه شروع بسیاری از روشهای بیولوژی مولکولی، ضرورت جداسازی DNA با کیفیت عالی است. معمولا کیفیت DNA با عواملی از قبیل عدم آلودگی ناشی ازRNA ، پروتئین، لیپید و سایر ساختارهائی که برای آنزیمهای برشی و پلی مرازها مزاحمت ایجاد میکنند سنجیده میشود. معمولا DNA استخراج شده از بلوک های پارافین و بافتهای فیکس شده در فرمالین بخاطر اثرات تخریبی وارد شده بر DNA، از کی...
sperm dna is known to contribute one half of the genomic material to the offspring. the integrity of sperm dna is important in fertilization, embryonic and fetal development, and postnatal child well being. the nature has created multiple barriers that allow only the fittest sperm to reach and fertilize an oocyte. however, assisted reproductive techniques (art), like ivf and icsi, may allow spe...
The presence of organic compounds and high amount of secondary metabolites (polysaccharides, phenolic component, etc.) in mosses cause difficulties in DNA extraction that are followed by problems in PCR reactions. In lower plants, various methods have been used for DNA extraction including silica gel and different commercial kits. These methods mostly use hazardous (like phenol or liquid nitrog...
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